Improved Green-emitting Fluorescent Proteins

The advent of fluorescent proteins (FPs) has redefined the use of fluorescence microscopy in every biological discipline. Green fluorescent protein (GFP) and its variants are commonly used in celluar imaging studies. Recently, a novel fluorescent protein, named vivid Verde Fluorescent Protein (VFP), was isolated from Cyphastrea microphthalma, a scleractinian coral found in the warmer waters of the Australian Great Barrier Reef. The VFP sequence was modified for optimal fluorescence for use in a variety of molecular and biological applications.